Nanopore sequencing in non-human forensic genetics
نویسندگان
چکیده
منابع مشابه
Forensic SNP Genotyping using Nanopore MinION Sequencing
One of the latest developments in next generation sequencing is the Oxford Nanopore Technologies' (ONT) MinION nanopore sequencer. We studied the applicability of this system to perform forensic genotyping of the forensic female DNA standard 9947 A using the 52 SNP-plex assay developed by the SNPforID consortium. All but one of the loci were correctly genotyped. Several SNP loci were identified...
متن کاملNanopore Sequencing: A Survey
A single molecule of Deoxyribonucleic acid (DNA) is being sequenced by using an innovative concept of Nanopore. The orientation of nanopore define the channel of ions through the pore comes into contact with nucleotide, the permutation and the transition kinematics of each molecule of DNA. The divergence of electrical signal observed when the four chemical bases adenine (A), guanine (G), cytosi...
متن کاملPCR in forensic genetics.
Since the introduction in the mid-1980s of analyses of minisatellites for DNA analyses, a revolution has taken place in forensic genetics. The subsequent invention of the PCR made it possible to develop forensic genetics tools that allow both very informative routine investigations and still more and more advanced, special investigations in cases concerning crime, paternity, relationship, disas...
متن کاملThe evolution of nanopore sequencing
The "$1000 Genome" project has been drawing increasing attention since its launch a decade ago. Nanopore sequencing, the third-generation, is believed to be one of the most promising sequencing technologies to reach four gold standards set for the "$1000 Genome" while the second-generation sequencing technologies are bringing about a revolution in life sciences, particularly in genome sequencin...
متن کاملThree decades of nanopore sequencing
Received 6 January 2015; accepted 5 November 2015; published online 6 May 2016; doi:10.1038/nbt.3423 and together with Sergey Bezrukov at the US National Institutes of Health (NIH), had established the conditions that were necessary to avoid spontaneous gating (pore closure) of the a-hemolysin channel3. This was important, because spontaneous gating would have hindered, or confused, observation...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Emerging Topics in Life Sciences
سال: 2021
ISSN: 2397-8554,2397-8562
DOI: 10.1042/etls20200287